The genus Aspergillus covers a group of filamentous fungi that includes saprophytes, human and plant pathogens as well as species being exploited in biotechnology. If the frequency of specific GO terms in a list of genes or proteins is higher than expected by chance, it is likely that these enriched GO terms are related to the biological processes under investigation. It has been used in many studies to highlight major trends in genomic, transcriptomic or proteomic datasets and describe them with a controlled vocabulary. Enrichment analysis of GO terms is a well accepted approach to dissecting omics data in a non-biased manner. As a consequence, the specificity of GO terms increases with increasing distance from their root node. GO terms are organized as directed acyclic graphs (DAG) meaning that GO terms are connected as nodes by directed edges defining hierarchical parent-child relationships. It comprises Biological Process (BP), Molecular Function (MF) and Cellular Component (CC) ontologies. Gene Ontology (GO) is a framework for functional annotation of gene products aiming to provide a unique vocabulary for living systems. Furthermore, we have given a general example of how a well annotated genome can help improving GO annotation of related species to subsequently facilitate the interpretation of omics data. By depositing the newly mapped GO annotation online as well as integrating it into the web tool FetGOat, we provide new, valuable and easily accessible resources for omics data analysis and interpretation for the genus Aspergillus. nidulans GO annotation to seven other Aspergilli. To demonstrate the value of those new resources for functional analysis of omics data for the genus Aspergillus, we performed two case studies analyzing microarray data recently published for A. Both the annotation files and the web application FetGOat are accessible via the Broad Institute's website ( ). To further improve their accessibility, we developed a web application for GO enrichment analysis named FetGOat and integrated GO annotations for all Aspergillus species with public genome sequences. GO annotation files compatible with diverse publicly available tools have been generated and deposited online. nidulans genes to at least one of seven other Aspergillus species: A. Resultsīased on protein homology, we mapped 97% of the 3,498 GO annotated A.
#Cytoscape and blast2go manual#
However, GO annotation based on both computational predictions and extended manual curation has so far only been available for one of its species, namely A. The genus Aspergillus comprises fungal species that are important model organisms, plant and human pathogens as well as industrial workhorses. As such, Gene Ontology (GO) annotation has become a well accepted framework for functional annotation. Detailed and comprehensive genome annotation can be considered a prerequisite for effective analysis and interpretation of omics data.
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